Funding for this research was provided by:
Fundação de Amparo à Pesquisa do Estado de São Paulo (#2009/16118-5, #2015/16850-9)
Received: 1 November 2018
Accepted: 12 June 2019
First Online: 25 June 2019
Ethics approval and consent to participate
: All experimental procedures were approved by Ethics Committee of the School of Agricultural and Veterinarian Sciences, São Paulo State University (UNESP), Jaboticabal, SP, Brazil (protocol number 18.340/16). The animals were provided by Qualitas Nelore breeding program company and they were slaughtered in commercial slaughterhouses. These slaughterhouses have animal welfare departments staffed by professionals trained by WAG (World Animal Protection) to ensure that the animals are killed humanely using a captive bolt pistol for stunning.
: Not applicable.
: The authors declare that they have no competing interests.