Tretina, Kyle
Pelle, Roger
Orvis, Joshua
Gotia, Hanzel T.
Ifeonu, Olukemi O.
Kumari, Priti
Palmateer, Nicholas C.
Iqbal, Shaikh B. A.
Fry, Lindsay M.
Nene, Vishvanath M.
Daubenberger, Claudia A.
Bishop, Richard P.
Silva, Joana C. http://orcid.org/0000-0001-6502-7026
Funding for this research was provided by:
National Institute of Allergy and Infectious Diseases (AI007540-14)
Bill and Melinda Gates Foundation (OPP1078791)
Agricultural Research Service (59-5348-4-001)
Article History
Received: 27 August 2019
Accepted: 18 March 2020
First Online: 3 April 2020
Ethics approval and consent to participate
: This study was carried out in strict accordance with the recommendations in the standard operating procedures of the Institutional Animal Care and Use Committee (IACUC) of the International Livestock Research Institute (ILRI), in Nairobi, Kenya. ILRI’s IACUC was established in 1993, and the institute complies voluntarily with the UK’s Animals (Scientific Procedures) Act 1986 (ExternalRef removed) that contains guidelines and codes of practice for the housing and care of animals used in scientific procedures. Schizont-infected lymphocyte cultures were derived from lymph node biopsies taken from cattle experimentally infected with <i>T. parva</i> sporozoite stabilates, as described in Morzaria et al. [CitationRef removed]. The studies in which cattle were infected were specifically approved by ILRI’s IACUC. The expansion of the infected lymphocyte cultures, conducted to generate the material used in this study, does not necessitate explicit IACUC approval.
: Not applicable.
: The authors declare that they have no competing interests.