Ma, Jun-Lei
Qiang, Jun
Tao, Yi-Fan
Bao, Jing-Wen
Zhu, Hao-Jun
Li, Lian-Ge
Funding for this research was provided by:
Central Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences (NO. 2019JBFC01; 2020TD37)
Article History
Received: 29 April 2020
Accepted: 27 January 2021
First Online: 6 February 2021
Ethics approval and consent to participate
: The experimental protocols were approved by the Institutional Animal Care and Use Committee of Nanjing Agriculture University (Nanjing, China). The GIFT were maintained in well-aerated water and deep anesthetized by treating with 100 mg L<sup>− 1</sup> MS-222 (Argent Chemical Laboratories, Redmond, WA, USA). The GIFT were euthanized using rapid cooling method by soaking the experimental fish in 30 L 0 °C ice water mixture (25 L of ice and 5 L of water). The livers were extracted from GIFT based on the Guide for the Care and Use of Laboratory Animals in China.
: Not applicable.
: The authors declare no conflicts of interest.