Gu, Qianhong
Wang, Shi
Zhong, Hui
Yuan, Hui
Yang, Junliu
Yang, Conghui
Huang, Xuexue
Xu, Xiaowei
Wang, Yude
Wei, Zehong
Wang, Jing
Liu, Shaojun
Article History
Received: 9 June 2021
Accepted: 14 March 2022
First Online: 28 March 2022
Declarations
:
: We confirm that all methods were carried out in accordance with relevant guidelines and regulations. All methods are reported in accordance with ARRIVE guidelines (ExternalRef removed). In this study, many genetic data were obtained through the Genbank (see Additional files InternalRef removed, InternalRef removed, InternalRef removed), while some samples (homoploid 2nNCRC, <i>Cyprinus carpio</i> and <i>Megalobrama amblycephala</i>) used in this study were cultured in ponds at the State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, and fed adequate artificial feed. Before taking pterygiophore and muscle tissue, the fish were deeply anaesthetized with 100 mg/L MS-222 (Sigma-Aldrich, St. Louis, MO, USA) prior to dissection. And they were snap-frozen in liquid nitrogen and stored at − 80 °C before use. Fish work was performed in strict accordance with the recommendations in the Guidelines for the Care and Use of Laboratory Animals of the National Advisory Committee for Laboratory Animal Research in China and approved by the Animal Care Committee of Hunan Normal University (Permit Number: 4237).
: Not applicable.
: The authors declare that they have no competing of interests.