Zhao, Juan-Juan
Sun, Xin-Yu
Tian, Sai-Ning
Zhao, Zong-Ze
Yin, Meng-Di
Zhao, Mei
Zhang, Feng
Li, Si-Ang
Yang, Zhi-Xue
Wen, Wei
Cheng, Tao
Gong, An
Zhang, Jian-Ping
Zhang, Xiao-Bing
Funding for this research was provided by:
National Key Research and Development Program of China (2019YFA0110803)
National Natural Science Foundation of China (81870149)
Chinese Academy of Medical Sciences (CAMS) Innovation Fund for Medical Sciences (2022-I2M-2-003)
Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences (2020-PT310-011)
Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project (TSBICIP-KJGG-017)
CAMS Fundamental Research Funds for Central Research Institutes (3332021093)
Haihe Laboratory of Cell Ecosystem Innovation Fund (HH22KYZX0022)
State Key Laboratory of Experimental Hematology Research Grant (Z23-05)
Article History
Received: 5 June 2023
Accepted: 24 January 2024
First Online: 17 February 2024
Declarations
:
: The mice were maintained at the State Key Laboratory of Experimental Hematology (SKLEH, Tianjin, China). All animal experiments complied with protocols approved by the Institutional Animal Care and Use Committee of SKLEH and the Institute of Hematology. The in vivo animal experiments were designed and reported following ARRIVE guidelines (ExternalRef removed). Euthanasia of the mice was performed using inhalation anesthesia followed by dislocation of the cervical vertebrae. Initially, the mice were placed in an induction chamber and inhaled isoflurane. The anesthetic gas concentration was carefully adjusted to ensure complete unconsciousness and lack of response to external stimuli. Subsequently, the cervical vertebrae were swiftly and completely dislocated to minimize any potential pain or distress to the mice.
: Not applicable.
: The authors declare no competing interests.