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Article History

Received: 8 October 2019

Accepted: 24 February 2020

First Online: 4 March 2020

Ethics approval and consent to participate

: Participation in the study by the farmers was voluntary and collection of samples was done after explaining the project fully to the farmers and obtaining verbal consent. The animals that were slaughtered were purchased from the farmers at the agreed price and a consent form was signed by the farmer. Ethics permission to collect animal samples was obtained from the University of Pretoria, Animal Ethics Committee (ref: <b>V078–16, VO50–16,V136–16</b>) and the Department of Agriculture Forestry and Fisheries (DAFF) under the Section 20 (ref: 12<b>/11/1/1/6/1</b>). Animal samples were also collected and submitted by the State Veterinary Officers as mandated by the National Department of Agriculture, land Reforms and rural Development for TB diagnostic testing of animals according to the animal disease legislation act 35 of 1984 for bovine tuberculosis control in South Africa.

: Not applicable.

: The authors declare that they have no competing interests.