Funding for this research was provided by:
Gun och Bertil Stohnes Stiftelse
H2020 Marie Skłodowska-Curie Actions (676144)
Margareta af Ugglas Stiftelse
Stiftelsen för Gamla Tjänarinnor
O. E. och Edla Johanssons Vetenskapliga Stiftelse
Progretto di Ricerca di Interesse Nazionale (PRIN2015N4FKJ4)
Received: 20 August 2019
Accepted: 27 January 2020
First Online: 4 February 2020
Ethics approval and consent to participate
: The use of human brain material in this study was conformed to the Declaration of Helsinki and approved by the by the regional ethical review board of Stockholm (2015/1803–31/2). Fresh frozen brain tissue from hippocampus of one AD and one control subject and cortex of three AD and three control subjects was obtained from the Harvard Brain Tissue Resource Center at the NIH Brain Bank, US who states that “All brain tissue is procured, stored, and distributed according to applicable state and federal guidelines and regulations involving consent, protection of human subjects and donor anonymity”.All animals used in this study were handled according to the Swedish, German or Italian national guidelines and the guidelines of Karolinska Institutet, the University of Göttingen and the University of Milano as well as the current European Law (Directive 2010/63/EU). Male Wistar/Sprague–Dawley rats (Charles River) were sacrificed either directly by cervical dislocation or carbon dioxide, followed by decapitation or by first anesthetizing the rats with isoflurane (3–4%, inhalation for 2–3 min) followed by euthanization with carbon dioxide. The use of rat brain in this study was approved by the Animal research ethical committee of southern Stockholm (S21-14) and the Italian Ministry of Health (#326/2015).
: We do not have any information from the NIH Brain Bank whether a consent to publication have been provided but as stated above, donor anonymity is guaranteed and it therefore not possible to trace back to the patients. The patients are also deceased.
: The authors declare that they have no competing interests.