Funding for this research was provided by:
Department of Biotechnology , Ministry of Science and Technology
Received: 15 November 2016
Accepted: 6 March 2018
First Online: 13 March 2018
Ethics approval and consent to participate
: Immortalized normal human breast epithelial cell line MCF10A, and the human breast cancer MCF-7 and MDA-MB-231 cells were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA) and collected from cell repository of Rajiv Gandhi Centre for Biotechnology. The pre-malignant and in situ grade cell lines-MCF10AT and MCFDCIS were a kind gift from Prof.Suresh Kumar Rayala (Indian Institute of Technology-Madras, India). Human breast tissue samples were collected from Regional Cancer Centre (RCC), Thiruvananthapuram, India after obtaining approval from the Institutional Human Ethics Committee HEC No. 42/2008, Regional Cancer Centre, Thiruvananthapuram. The samples were collected from patients who underwent primary surgery without prior chemotherapy or radiotherapy. Informed written consent was taken from all the patients prior surgery/excision of tissue.
: Not applicable.
: The authors declare that they have no competing interests.
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