Funding for this research was provided by:
National Eye Institute (R01EY024140, R01EY025947, P30EY27125)
Presbyterian Health Foundation
Research to Prevent Blindness
Received: 8 September 2017
Accepted: 3 April 2018
First Online: 16 April 2018
: This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The protocol was approved by the Institutional Animal Care and Use Committee of the University of Oklahoma Health Sciences Center (protocol number 16–086). Six week old C57BL/6 J (wild type) mice were acquired from the Jackson Laboratory (Catalog 000664, Bar Harbor ME) and age-matched, homozygous TLR4−/− mice on the C57BL/6 J background (Eric Perlman, Case Western University, with the permission of S. Akira). Mice were allowed to adjust to conventional housing 2 weeks prior to injection to equilibrate their microbiota. Mice were anesthetized with a cocktail of 85 mg ketamine/kg and 14 mg xylazine/kg prior to injections of bacteria. Mice were euthanized by CO2 inhalation.
: The authors declare that they have no competing interests.
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