Han, Wenrui
Jiao, Yao
Mi, Sicong
Han, Shu
Xu, Junji
Li, Song
Liu, Yi
Guo, Lijia
Funding for this research was provided by:
Song Li (2022-2-2142)
Yi Liu (81991504)
Lijia Guo (2022YFC2504200)
Article History
Received: 12 February 2023
Accepted: 14 July 2023
First Online: 10 August 2023
Declarations
:
: We followed the Basel Declaration and the International Council for Laboratory Animal Science (ICLAS) ethical guidelines and relevant guidelines of Directive 2010/63/EU for animal care and use. The animal experiment was conducted according to ARRIVE guidelines, and the experimental protocols were approved by the Animal Care and Use Committee of the Beijing Stomatological Hospital, Capital Medical University, Beijing, China (KQYY-201907–003). Our experimental design follows the 3R principle, the grouping of animals, calculation of sample size, and the experimental procedures have been given, and the anesthesia and euthanasia methods were consistent with the American Veterinary Medical Association (AVMA) Guidelines for the Euthanasia of Animals (2020). During the experiment, the experimental animals were kept in controlled laboratory settings of temperature 22–25 °C, good ventilation, and regular light/dark cycle (12/12 h).
: Not applicable.
: All the authors declare no competing interests.