Funding for this research was provided by:
Fundação de Amparo à Pesquisa do Estado de São Paulo (grant #2016/03424-1)
PROPe-UNESP (Scholarship #668, grant #668)
National Counsel of Technological and Scientific Development (#830071/2000-8)
Ministério da Ciência e Tecnologia - Ensino Superior e Técnico Profissional
Received: 3 May 2017
Accepted: 8 February 2018
First Online: 14 February 2018
Ethics approval and consent to participate
: All volunteers received an explanation about the study and consented to donated saliva for pellicle formation by signing an informed consent term. The study was approved by the Institutional Ethical Committee at São Paulo State University (Unesp), School of Dentistry, Araraquara. (CAAE: 31.725.114.8.0000.5416).
: “Not applicable”. Saliva samples were pooled without volunteer identification.
: The authors declare that they have no competing interests.
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