Received: 17 July 2021
Accepted: 27 July 2022
First Online: 26 August 2022
: Male C57BL/6 J mice aged 8 weeks (<i>n</i> = 32) were obtained from SPF Biotechnology Co., Ltd. (Beijing, China). The mice were raised under the following conditions: 12/12 h light/dark cycles, temperature of 23 + 2 °C, and relative humidity of 55%, at the Tianjin Eye Institute; food and water were provided freely. The mice were randomly divided into four groups: control (<i>n</i> = 8), light-damaged (<i>n =</i> 8), low-dose chrysanthemum extract (<i>n =</i> 8), and high-dose chrysanthemum extract (<i>n =</i> 8) groups. The concentration ranged from 0.23 g/kg/day (low dose) to 0.38 g/kg/day (high dose). Intragastric administration (0.2 mL/day) was performed continuously for 8 weeks. All procedures adhered to the International Standards for the Care and Use of Laboratory Animals, principles and guidelines of the Chinese Council of Animal Care, and the experimental protocol was approved by Institutional Animal Care and Use Committee of Tianjin Medical University. Prior to being subjected to experimentation or sacrifice, the mice received isoflurane inhalation to minimise suffering. All methods were reported in accordance with the ARRIVE guidelines (ExternalRef removed).
: Not applicable.
: The authors declare that they have no competing interests.