Abdu, Hussen
Ergete, Wondwosen
Tadele, Ashenif
Woldekidan, Samuel
Abebe, Abiy
Seyoum, Girma
Article History
Received: 6 September 2022
Accepted: 23 March 2023
First Online: 3 April 2023
Declarations
:
: The study was conducted after getting a letter of ethical approval from the Department Graduate Committee (DGC) (Protocol number 084/18/ANAT) of the Anatomy Department and the Institutional Review Board (IRB) of the College of Health Sciences, Addis Ababa University (Form AAUMF 03-008, Elements Reviewed AAUMF 01-008). The plant leaves were collected following institutional and local guidelines of EPHI. In addition, the animals were kept to the highest standards for the humane use of animals in the biomedical research laboratory of EPHI. The rats were not subjected to any needless, painful, or frightening manipulations. The extract was administered by an expert and maximum effort was applied to prevent contamination and the introduction of pathogens to the animals. Before being sacrificed, to avoid pain and suffering the rats were anesthetized with pentobarbital. Lastly, sacrificed rats and unused fetuses were disposed of humanely following the laboratory standards of EPHI.
: Not applicable.
: The authors declare no competing interests.