Funding for this research was provided by:
Fondo Nacional de Desarrollo Científico y Tecnológico (1171307, 1171318)
Fondo de Financiamiento de Centros de Investigación en Áreas Prioritarias INCAR (15110027)
Received: 23 May 2019
Accepted: 7 August 2020
First Online: 17 August 2020
Ethics approval and consent to participate
: A group of healthy pathogen-free rainbow trout (7.24 ± 1.35 g body weight [Mean and SD], <i>n</i> = 160) were obtained from “Río Blanco Federico Albert Taupp” fish farm (Valparaíso Region), a hatchery with no history of disease (located in central Chile, Los Andes). This hatchery has the certificate of being free of pathogens included in Chilean List 1, 2 and 3. In addition, before moving fish, by sanitary regulations, fish were certificated as free of any pathogen described in Chile. All the experimental procedures and animal manipulations were designed and approved according to the ethical handling of live animals’ standards from the Chilean National Commission of Scientific and Technological Research (CONICYT), and the Ethics Committee for Animal Experiments of the Universidad Andres Bello.
: Not applicable.
: The authors declare that they have no competing interest.