Received: 26 November 2020
Accepted: 14 April 2021
First Online: 1 May 2021
: This study was approved by the University of Saskatchewan Animal Research Ethics Board and adhered to the Canadian Council on Animal Care guidelines for humane animal use (Protocols 20110102 and 20160023). The infected pregnant gilts were closely monitored for clinical signs and a human intervention point checklist was established to monitor the gilts for critical conditions post-inoculation. It was not feasible to monitor fetal conditions after the viral inoculation, but fetal compromise death was expected to occur at the later time points. Gilts were euthanized with intravenous sodium pentobarbital overdose followed by cranial captive bolt and exsanguination. Fetuses were euthanized as humanely as possible given that pentobarbital sodium crosses the MFI rapidly and enters fetal circulation prior to the onset of hypoxia and asphyxia.
: not applicable.
: The authors declare no conflicts of interest with respect to the research, authorship and/or publication of this article.