Funding for this research was provided by:
Uniwersytet Przyrodniczy we Wroclawiu (D220/0002/18)
Received: 31 March 2020
Accepted: 7 January 2021
First Online: 15 January 2021
Ethics approval and consent to participate
: In line with the Act of 15 January 2015 on the protection of animals used for scientific and educational purposes (Journal of Laws of 2015, item 266), the restrictions defined in the Act shall not apply to: 1. healthcare facilities for animals as defined in the Act of 18 December 2003 on healthcare facilities for animals (Journal of Laws of 2004, No. 11, item 95 as amended), as well as agricultural activity, raising and breeding livestock, not intended for carrying out medical procedures, as defined in the Animal Protection Act; 2. clinical veterinary studies carried out in accordance with Article 37ah-37ak of the Act of 6 September 2001, Pharmaceutical Law (Journal of Laws from 2008, No. 45, item 271, as amended); 3. activity aimed at identifying animals; 4. capturing wild animals for biometric and systematic assessment; 5. veterinary procedures which to not cause pain, suffering, distress, or permanent health impairment equal to or more invasive than the insertion of a needle. Hence, the current study did not require the approval of the Ethics Committee. Procedures performed in healthy horses were in accordance with the approval of the 2nd Local Ethics Committee for Animal Experiments in Wroclaw (permission No 86/2018).
: In privately owned horses, owner’s informed consent to study participation and publication of its results was obtained prior to commencement of any study-related procedures.
: The authors declare that they have no competing interests.