Funding for this research was provided by:
Associazione Italiana per la Ricerca sul Cancro (14318 and 18812, IG21431)
Ministero della Salute (RF-2016-02362946)
Fondazione Cariplo (2015-0901)
Received: 31 March 2021
Accepted: 12 July 2021
First Online: 21 July 2021
: Plasma samples were collected from high-risk heavy-smoker volunteers (age 50–75 years), including current or former smokers with a minimum pack/year index of 30 enrolled in a LDCT screening trial performed at our institution (BioMild Trial, ClinicalTrials.gov: NCT02247453)[CitationRef removed]. EVs were also isolated from the plasma of lung cancer patients affered to Thoracic Unit of our Institute. Plasma collection was approved by the Internal Review and the Ethics Boards of the Istituto Nazionale Tumori (INT 11–21) of Milan. All patients provided informed consent.
: All authors involved in the study had given their consent for submitting this article for publication.
: The authors declare no conflicts of interest.