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Article History

Received: 4 August 2018

Accepted: 26 December 2018

First Online: 8 January 2019

Ethics approval and consent to participate

: Research on human subjects was conducted in compliance with existing regulations relating to the protection of human subjects and was evaluated and approved (#IRB-15-6664) by the Institutional Review Board/Ethics Review Committee at The Scripps Research Institute. Clinical samples were obtained from the Florida Department of Health (DOH) and Antibody Systems Inc. Samples collected in Florida were collected under a waiver of consent granted by the Florida DOH Human Research Protection Program. The work received a non-human subjects research designation (category 4 exemption) by the Florida DOH since this research was performed with leftover clinical diagnostic samples involving no more than minimal risk. Hence, written informed consent was not obtained. All samples were de-identified prior to receipt by the study investigators. The experimental methods used comply with the Helsinki Declaration.Research involving Indian origin rhesus macaques was conducted at the California National Primate Research Center, and experimental infections of mice upon which <i>Ae. aegypti</i> mosquitoes fed were performed at the University of California, Davis, School of Veterinary Medicine. Both institutes are fully accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care International. Animals were cared for in accordance with the National Research Council Guide for the Care and Use of Laboratory Animals and the Animal Welfare Act. Animal experiments were approved by the Institutional Animal Care and Use Committee of UC Davis (protocols #19211 and #19695 for rhesus macaques, protocol #19404 for mice). All macaques samples used in this study were from approved studies [CitationRef removed]; and none were generated specifically for this work.

: Not applicable.

: NJL has received travel and accommodation expenses from Oxford Nanopore Technologies to attend meetings, and an honorarium to speak at an internal company meeting. NJL has previously received free-of-charge reagents and consumables in support of research projects from Oxford Nanopore Technologies. The other authors declare that they have no competing interests.

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