Funding for this research was provided by:
Japan Agency for Medical Research and Development (JP16gm0510005h0006, JP19am0101105)
Japan Society for the Promotion of Science (17H06331, JP18H05527)
Received: 14 August 2019
Accepted: 3 December 2019
First Online: 19 December 2019
Ethics approval and consent to participate
: Human clinical specimens were prepared from discarded tissue during surgery under consent of donors at Saitama Medical University International Medical Center according to the Institutional Review Board (IRB) protocol 15-209, at Ohta Memorial Hospital according to the IRB protocols 068 and 069 and at The University of Tokyo according to the IRB protocol G3577. Primary ECs were isolated at The University of Tokyo according to the IRB protocol 17-311. Other ECs were prepared from a commercial biobank (Lifeline Cell Technology, Frederick, MD). DNA and RNA samples were prepared at the University of Tokyo according to the IRB protocol 12-81.
: As to purchased cells, not applicable. As to primary cultivated cells from human tissue, written informed consent was obtained from the patients for publication of their individual details and accompanying images in this manuscript. The consent form is held by the authors and is available for review by the Editor-in-Chief.
: The authors declare that they have no competing interests.