Armaka, Marietta http://orcid.org/0000-0003-0985-9076
Konstantopoulos, Dimitris http://orcid.org/0000-0003-2142-3021
Tzaferis, Christos http://orcid.org/0000-0002-8940-5359
Lavigne, Matthieu D. http://orcid.org/0000-0003-1543-8745
Sakkou, Maria http://orcid.org/0000-0003-3616-3277
Liakos, Anastasios http://orcid.org/0000-0001-8509-3768
Sfikakis, Petros P. http://orcid.org/0000-0001-5484-2930
Dimopoulos, Meletios A. http://orcid.org/0000-0001-8990-3254
Fousteri, Maria http://orcid.org/0000-0003-1729-5899
Kollias, George http://orcid.org/0000-0003-1867-3150
Funding for this research was provided by:
H2020 European Research Council (340217)
European Research Council (309612)
Hellenic Foundation for Research and Innovation (3780)
Stavros Niarchos Foundation
Foundation for Research in Rheumatology (#061)
Article History
Received: 18 January 2022
Accepted: 30 June 2022
First Online: 26 July 2022
Declarations
:
: All animal experiments have been performed in accordance with the guidance of the IACUC of BSRC “Alexander Fleming” and in conjunction with the Veterinary Service Management of the Hellenic Republic Prefecture of Attika/Greece (license number: #2199-11/4/2017). Experiments were monitored and reviewed throughout their duration by the respective Animal Welfare Body for compliance with the permission granted. The mice were housed in specific pathogen-free facilities, reared under conditions that conform to national animal research regulations, under the following conditions: room temperature, 22 ± 1 °C; U. R, 55 ± 10%; 12/12 light/dark cycle; and cage size 335 cm<sup>2</sup> minimum according to the recommendation 2007/526 EC. The microbiological quality of animals is monitored according to the health monitoring recommendations and guidelines of the FELASA (ExternalRef removed). Animals were euthanized by CO<sub>2</sub> narcosis (or cervical dislocation performed by well-trained personnel), at indicated time points. This is the standard method recommended by the FELASA and AVMA. Other criteria for euthanasia included difficulty in breathing, weight loss over 20%, lethargy, dehydration, anorexia, hunched posture, inability to move, body temperature under 36.5 °C, and others (Directive 2010/63/EU of the European Parliament and of the Council Decision (September 22, 2010).Our analyses with datasets derived from human SFs were based on publicly available data that have been approved by relevant review boards. The datasets were requested from NCBI dbGaP Program datasets (enlisted as project #23790).
: Not applicable.
: The authors declare that they have no competing interests.