Funding for this research was provided by:
Conselho Nacional de Desenvolvimento Científico e Tecnológico (123028/2018-3)
Fundação de Amparo à Pesquisa do Estado de São Paulo (2011/05932-3, 2017/26075-8)
Received: 29 March 2021
Accepted: 10 July 2021
First Online: 27 July 2021
: The study was carried out in compliance with the ARRIVE Guidelines and according to the Arouca Law (nº 11.794/ October 8, 2008) and the Normative Resolutions of the National Council for Animal Experiments Control – CONCEA, both regulated by Brazilian Federal Constitution. All experiments in this manuscript were approved by the Institutional Ethics Committee on Animal Use (CEUA n. 5541040218) of the Federal University of Sao Paulo (UNIFESP), Brazil. Male C57Bl/6 mice were obtained from the Center for Development of Animal Models for Medicine and Biology (CEDEME, Federal University of São Paulo). They were kept from 3 to 9 months of age at the animal house of the Department of Bioscience in a temperature-controlled room (22 °C) with a 12:12-h light-dark cycle (7:00–19:00 h) and free access to water and AIN-93 M diet (Rhoster Indústria e Comércio LTDA, São Paulo, Brazil). At the end mice were anesthetized with a mixture of ketamine (75 mg/Kg), xylazine (10 mg/kg), fentanyl (0.5 mg/Kg) and acepromazine (1 mg/Kg) administered intraperitoneally and euthanized by cardiac perfusion.
: Not applicable.
: The authors declare that they have no competing interests.