Funding for this research was provided by:
Agricultural Research Service (5030-32000-116-00-D, 5030-32000-221-00-D)
Received: 26 October 2020
Accepted: 4 January 2021
First Online: 21 January 2021
Ethics approval and consent to participate
: Blood samples collected from healthy cows were used to isolate peripheral blood mononuclear cells (PBMCs) to generate monocyte-derived macrophages (MDMs) via cell culture. The animals were handled in accordance with the Animal Welfare Act, and all procedures were approved by the Institutional Animal Care and Use Committee at the National Animal Disease Center, Ames, IA.
: The authors declare that they have no competing interests.